Custom Polyclonal Antibody Services
Antibodies (also known as immunoglobulins, abbreviated (Ig) are gamma globulin proteins that are found in blood
or other
bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign objects, such
as bacteria and viruses. As an antigen is injected into the mammal, it induces the B-lymphocytes to produce
IgG immunoglobulins specific for the antigen. The unique part of the antigen recognized by an antibody is called
the epitope. These epitopes bind with their antibody in a highly specific interaction. Polyclonal antibodies (or
antisera) are antibodies that are obtained from different B cell resources. They are a combination of immunoglobulin
molecules secreted against a specific antigen, each identifying a different epitope. Due to polyclonal antibodies
recognize multiple epitopes on any one antigen, cause agglutination and precipitation reactions, it is widely used
in the field of immunological diagnosis Polyclonals can help amplify signal from target protein with low expression
level, as the target protein will bind more than one antibody molecule on the multiple eptitopes. Due to recognition
of multiple epitopes, polyclonals can give better results in IP / ChIP .Polyclonal antibodies are often the preferred
choice for detection of denatured proteins.
A reliable immunological assay demands reliable antibodies and antibody services. Our custom antibody production
services include recombinant protein expression, peptide design and synthesis, polyclonal antibody production in
various animals such as Rabbit、Mouse、Rat、Goat and Chicken, affinity purification, conjugation, fragmentation,
and immunoassay development. Youke Biotech’s technology team can supplies our customers the most relieable
and comprehensive antibody package services to ensure fast, economical and high quality poly-clonal antibody
production.
|
Service
|
Brief Description
|
Time
|
|
protein → anti-sera
|
antigen QC，immunize，
ELISA，bleed
|
60 days
|
|
protein → antibody
（protinA purification）
|
antigen QC，immunize，
ELISA，Bleed，ProteinA purify
|
70 days
|
|
protein→antibody
(antigen affinity purification)
|
antigen QC，immunize，
ELISA，bleed，antigen affinity purify
|
70 days
|
|
peptide → anti-sera
|
peptide conjugate，immunize，
ELISA，Bleed
|
70 days
|
|
peptide → antibody
（protinA purification）
|
peptide conjugate，immunize，
ELISA，bleed，proteinA purify
|
80 days
|
|
peptide → antibody
（antigen affinity purification）
|
peptide conjugate，immunize，
ELISA，bleed，antigen affinity purify
|
80 days
|
|
peptide sequence → anti-sera
|
peptide synthesis,peptide
conjugate，immunize，ELISA，bleed
|
80 days
|
|
peptide sequence →
antibody（protinA purification）
|
peptide synthesis,peptide conjugate,
Immunize,ELISA,bleed,
proteinA purify
|
90 days
|
|
peptide sequence → antibody
（antigen affinity purification）
|
peptide synthesis,peptide conjugate,
immunize,ELISA,bleed,antigen
affinity purify
|
90 days
|
|
peptide sequence with
phosphorylation site → antibody
|
peptides synthesis(phosphor peptide
and unmodified peptide )，
peptide conjugate，Immunize,ELISA,
Bleed，antigen affinity purify
(phosphor peptide and unmodified peptide)
|
100 days
|
|
peptide sequence with
methyl-specific site → antibody
|
peptides synthesis(methyl peptide
and unmodified peptide ),
peptide conjugate,Immunize,ELISA,Bleed,
antigen affinity purification
（methyl peptide and unmodified peptide）
|
100 days
|
|
protein sequence → antibody
（WB guaranted in overexpression
system）
|
peptide design,synthesis,
And conjugate;Immunize,ELISA,
Bleed,antigen affinity purify,
WB test(in overexpression system)
|
100 days
|
|
small molecules compound
→ anti-sera
|
hapten conjugate，immunize，ELISA，Bleed
|
75 days
|
|
small molecules compound
→ antibody（protinA purification）
|
hapten conjugate，immunize，ELISA，bleed，
proteinA purify
|
85 days
|
|
expression Plasmid → anti-sera
|
protein express and purify，eimmunize，ELISA，
bleed，WB
|
80 days
|
|
expression Plasmid →
antibody（protinA purification）
|
protein express and purify，eimmunize，ELISA，
bleed，WB,proteinA purify
|
90 days
|
|
cDNA → anti-sera
|
expression plasmid construct,protein express and purify，
eimmunize，ELISA，bleed，WB
|
100 days
|
|
cDNA → antibody
（protinA purification）
|
expression plasmid construct,protein express and purify,
eimmunize,ELISA,bleed,
WB,ProteinA,
|
110 days
|
|
DNA sequence → anti-sera
|
gene synthesis,expression plasmid construct,protein
express and purify,eimmunize,ELISA,bleed,WB
|
120 days
|
|
DNA sequence → antibody
（protinA purification）
|
gene synthesis,expression
plasmid construct,protein express
And purify,eimmunize,ELISA,bleed,
WB,proteinA purify
|
130 days
|
|
protein → anti-sera（goat）
|
antigen QC,immunize goat,ELISA,bleed
|
70 days
|
|
protein → anti-sera（chicken）
|
antigen QC,immunize chicken,ELISA,bleed
|
70 days
|
|
